The Influenza Primer Design Resource (IPDR) is designed to aid researchers in translating the vast amounts of influenza sequence information into highly effective influenza diagnostics. IPDR consists of a database of all influenza nucleotide sequences that is updated weekly and variety of bioinformatic analyses that aid in the development of primers and probes that can be used in diagnostic assays. After a user searches the database the sequences that were selected are aligned using the fast and accurate MAFFT algorithm. From the alignment the user is provided with a consensus sequence, based on user selected parameters, the percent conservation of each nucleotide for each position in the alignment, and the percentage of sequences representing each position of the alignment. Two additional analyses are performed on the consensus sequence(s). First the consensus sequence(s) is BLASTed against a database of primers and probes for influenza that have been in publications, identifying already established oligos that may be used. The consensus sequence(s) is also analyzed by Primer3 to determine potential oligos that may be used for the sequences of interests. These analyses make IPDR a valuable utility for quickly developing up to date influenza diagnostics.

Enter parameters below to search for influenza sequences. Use the CTRL key to select multiple items, or to deselect items. Leaving a field blank, will select all records for that parameter.